产品货号：
GS2154
中文名称：
Tru9I内切酶
英文名称：
Tru9I
产品规格：
300U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
技术规格
相关搜索：Tru9I内切酶，Tru9I限制性内切酶
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 65℃ in a total reaction volume of 50μl. |
| Recognition sites | T↑TAA |
| 来源 | E.coli strain that carries the cloned Tru9 I gene from Thermus ruber 9 |
| Assayed on | Lambda DNA |
| Optimal buffer | W?(10mMTris-HCl(pH 8.5 at 25℃);10mMMgCl2;100 mM NaCl;1 mM DTT.) |
| Optimal temperature | 65℃ |
| Storage conditions | 10mMTris-HCl-(pH 7.5);50 mM NaCl;0.1 mM EDTA;1 mM DTT;200 μg/ml BSA;50%glycerol Store at -20℃. |
| Ligations | After 20-fold overdigestion with enzyme more than 95%of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 40 u.a.of enzyme for 16hoursat 65℃. |
| Methlation effect | Blocked by TTAmA methylation. |
| Thermal inactivation | enzyme is inactivated by incubation at 80℃ for 20 mins. |
| Campatible Ends | MseI,SaqAI,Tru1I |
相关搜索：Tru9I内切酶，Tru9I限制性内切酶